Specifications General -Detection mode Fluorescence, Luminescence -Read method End point, kinetic. : 10 Mar 09 Page 1 of 14 Setup Guide on the BioTek Instruments FLx800 Fluorescence™ Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 Select option 3, ext. The BioTek Cytation cell imaging multimode microplate readers offer modularity to meet today’s workflows and are upgradable to meet future workflow requirements. 95. About. Users can choose to process all plates within the magazine or select a specific number. Lysin A constructs were expressed in M. plates can be used in the SPECTRAmax PLUS 384. Plate chamber design of the SpectraMax category 1 microplate readers. #3635 or Greiner cat. The Back Panel Figure 1. After mixing all the components, the absorbance of the mixture was continuously measured at 475 nm at 25°C using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA). Published Dec 16, 2020 | Updated Dec 16, 2020. Incubation. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Evans blue dye in the supernatant was detected at optical density (OD) 620 nm, using a SpectraMax M5 plate-reader (Molecular Devices, Sunnyvale CA). The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Information on how to reserve the plate reader Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. To monitor the aggregation kinetics, 10 μM ThT was added to a small aliquot (300 μL) of the mixture, and the fluorescence signal (λ ex = 444 nm, λ em = 485 nm) was monitored on a Spectramax M5 plate reader (Molecular Devices). How to export results files from the plate reader into Excel. Immunoblotting. Please contact Tatiana to reserve the plate reader time: [email protected] signals were scanned using a Molecular Devices SpectraMax M5 plate reader (M5, Molecular Devices, U. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. ScanLater Western Blot Detection Cartridge. 1) Select the correct plate and the wells that you want to measure. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). cerevisiae transcription factor Azf1p Taylor Stewart1, Benjamin E. Louis, MO) dissolved in PBS was injected over 30 seconds into the tail vein of a mouseIn bacteria, natural transposon mobilization can drive adaptive genomic rearrangements. 305 reference level saturation. White or black. This user guide provides safety information, warnings, cautions, tips, and instructions for operating the instrument, connecting cables, setting up the instrument, and troubleshooting. 3. The empty LNP matrix solution was made by mixing 300 μL of empty LNP solution with 45 mL diluent. The concentration of infectious virus in both stock. Standard curves were plotted using SoftMax® Pro Software (Figure 1). Lucifer yellow concentrations in the donor and acceptor wells were measured in the last of this incubation. Third this recommendation. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact [email protected]. Plates were incubated at 37°C for 6 hours and then equilibrated to room temperature for 30 min. 5. Prepare a 1:1 solution of Greiss Reagent 1:Greiss Reagent 2. After 30 min, the plate was washed with PBS 0. Temperature can be set to 4º above ambient to 45°C (thermometer icon) on main toolbar. 9000-0756. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. plates in 150 ll OS minimal medium (Schnider-Keel et al. After MMP9-antibody complex formation, substrate (20 μM for human and mouse assays, 10 uM for rat) was added and fluorescence was monitored in kinetic mode at 37°C on either a SpectraMax M2 or M5. Information on cell morphology, localization of signal, cell count, and more is obtained with Cytation 5 imaging mode. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. DH5α and BL21 (DE3) chemically competent E. 2. How-to-use guide for the Nanodrop Spectrophotometer. edu, 814-863-3503. RoHS Compliant. Thesupplyofhumidifiedair (zero air) was controlled by a solenoid valve with on and offLastly, Jurkat cells were least susceptible to Vorinostat treatment (all IC 50 > 50. SpectraTest ABS2 Absorbance Validation Package performance tests. 0 and room temperature. 72 MB. Then the plate was incubated at 37 °C for 2 h. Thus, Eu 3+ has a detection limit of approximately 10–100 amol nowadays depending upon. Figure 9. 5. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. PURPOSE. For a higher degreeTime Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. During the room temperature incubation (step 9. Reactions on 384-well plates (Corning) were monitored by fluorescence (emission 545 nm) using a SpectraMax M5 plate reader (Molecular Devices), with excitation at 274 nm, for 20 min at 2-min. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 5 pM (0. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The medium fluid was, respectively, collected at 4. Please contact Tatiana to reserve the plate reader time: tnl1@psu. 10569C 2 C 12 cells were treated exactly as described for free [Mg 2+] determination, except that MgG was replaced by 5 μM safranine O. SpectraMax M5e Multi-Mode Microplate Reader. The Molecular Devices Spectramax M5 Multi-Mode Plate Reader brings you the power and precision of a dedicated single mode plate reader, but with the space saving flexibility of 5 detection modes in one unit. High-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities. T o enable accurate quantita-tion, initially standard curves were plotted f or both Pi and. 3 Kg) Regulatory Regulatory CE and TUV marked. Plate Reader with 5 modes of detection, UV-Vis (Abs), Fluorescence (FI) , Lumi, TRF, F Polarization, with current validations, fully inspectedOpen the Gen 5 software, 2. 5. -well plate reader format. An emission wavelength of 535 nm was used for carboxy-H2DCFDA and 610 nm was used for DHE and MitoSOX ™. 3. Please contact Tatiana to reserve the plate reader time: tnl1@psu. Signals of wells containing only the target cells. Uncontrolled Copy for Training and Reference Purposes Only . Imagine a world where everyone smiles®. 1. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Cells were then pulsed with 1μCi 3 H-thymidine (Perkin Elmer, Waltham. There is currently no charge for using this plate reader. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. 25% (v/v) AlamarBlue reagent was then added to cells and incubated in normal cell culture conditions for 1 h, protected from light. The drawing shows a standard microplate of 15 mm height covered with a lid. 0200-0729. We use the SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA). : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. Fluorescein 0. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. The software is easy to use and contains settings for hundreds of commonly used assays, such as BCA and MTT. Plate Analysis 9. Flow cytometry analysis H22 cells (5 × 105 cells/well) were seeded in 6-well plates and then cells were treated with or without TB-TF (5, 10, 20, 40, 80 μg/mL) for 48 h. Attune Flow Cytometer; FacsCalibur; ImageQuant 800; Leica Cryostat; M5 Plate Reader; Odyssey CLx Imager; Typhoon 5;Then, the cells were replaced for incubation for 60 min. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 17. ". fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). Empirical Phase. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. Each plate reader or washer is calibrated, inspected, and lubricated—any potential issues are proactively addressed. The fluorescence was then measured with a fluorescence plate reader (SpectraMax M5; Molecular Devices, Inc. A standard curve was plotted using various numbers of live cells and used to calculate the bacterial viability (%) in the test samples. LbL films are immersed into 20 mM DTT in PBS buffer (2. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. 5. Perkin Elmer Victor X4 Multimode Plate Microplate Reader 2030-0040 with PC. Wide range of applications The VersaMax reader covers a wide range of applications:To monitor the increase rate, fluorescence measurement is then performed at 37°C at 10-s intervals for 10 min with Ex/Em 450/510 nm in the plate reader (Spectramax M5 plate reader, Molecular Devices, For each bacterial sample, we performed the reactions in duplicate and averaged the results. Products. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. The plate was read by a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm with 620 nm as reference. Enzyme activity from tissue homogenates was normalized to total protein content with the Bradford protein assay (Biorad). PDE-lux transcriptional fusion plasmids were constructed as previously described. 3). The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Most papers they cultured bacteria to OD600>0. After this time period, the plate was read for fluorescence intensity at ex/em wavelengths of 532/590 nm, on a SpectraMax M5 plate reader. It controls all the plate reader functions and offers powerful data analysis. com 9. Supersedes 1. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. 4-liter V-8 that sends power to all four wheels via the car’s. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. Supernatants were collected, and the concentration of l-lactate was determined by measuring absorbance of reaction samples using the Spectra Max M5 plate reader (Molecular Devices). In the M2, M5 and Plus 384, the light. When a feature or capability applies to only certain readers, this. SpectraMax Paradigm Multi-Mode Safety Guide fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). Select Start>Control panel>Device Manager. Bioluminescence assays showed that the engineered Luc-Cys was fully active when compared with 6 × His-NanoLuc prepared in our previous work (Zhang et al. Published Dec 16, 2020 | Updated Dec 16, 2020. com 10. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. The sample is typically placed in a cuvette through which light is sent horizontally. Absorbance readings were then taken at 650 nm using a Spectramax M5 plate reader with SoftMax Pro v5. 6), turn on the M5 Plate Reader and open the “BCA Template” file 9. plates with an 8-fold dispenser (Bio Tek Germany, Bad Friedrichshall, Germany) and the plates were then centrifuged for 1 min at 1,000 rpm and room temperature. The exact concentration of the RNA stock solution was calculated by UV absorption at 260 nm (measured by Spectramax M5 Plate Reader, Molecular Devices, San Jose, CA) with the accepted extinction coefficient of the RNA (21. Lastly, Jurkat cells were least susceptible to Vorinostat treatment (all IC 50 > 50. The ultrapure water used in the experiments was prepared with a Millipore Direct-Q® 3 system (Merck Millipore, MA, U. Riss, Germany) at 330 nm and room temperature for 30 min. HSL_EQ_004: Use and Maintenance of a BioTek Plate Washer in the HPV Serology Laboratory 3. After mixing with freshly diluted substrate (50 μl well −1), bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) under luminescence mode. BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. Following lysis, the plate was centrifuged at 500g for 3 min and supernatants transferred to a Maxisorp 96-Well Flat-Bottom Assay Plate (Nunc). The plate was washed and an anti-rabbit peroxidase-conjugated secondary antibody (Jackson Immunoresearch) was applied at a 1:3000 dilution in PBS. 2016 Aug 2; 7(31): 50027–50042. The Back Panel Figure 1. 7°C (gradient heating design). They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Developed with Promega reagents to provide a. 5°C at 37°C. The StakMax stacker also allows users to feed plates into the loading area manually. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . 0 . A solution of phenol red-free MEM containing 1. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only (blank) and four wells containing 50 nM ATP (sample). IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. 1. 4, step 4, will be used for analysis. Results were expressed as absorbance units. Release of free AMC by proteolytic activity was monitored on Molecular Devices M5 plate reader with excitation and emission wavelengths of 360 and 460 nm, respectively. 9. Select Start>Control panel>Device Manager. ELISAs were carried out according to the manufacturer’s instructions for IL-1β (BD Biosciences, San Diego, CA), TNFα, MIP-1α, and MCP-1 (R&D Systems, Minneapolis, MN) and quantified using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA). Nonspecific binding was determined by competition with 1. 3. Page 1 of 8 . Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. With optimized reagents, option to operate the. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Mutations in the α2–α2′ and α4–α4′ dimer interfaces affect the secondary structure of the DENV2C protein. Description. 2. 5. Clotting and lysis were monitored in a Spectramax M5 plate reader (Molecular Devices, Berkshire, United Kingdom) at 340nm (plasma) or 405nm (fibrinogen) and analyzed using online apps to determine times to 50% lysis. For Western blot analysis of apoptosis marker caspase-3, the equivalent total protein from control and SPM. 18632/oncotarget. Product Overview. Download : Download full-size image; Fig. 2. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax M-series Microplate readers. 025 fmol/well, 384-well plate) Quad monochromator: Fluorescein 2. S6. The % PEG midpt and apparent solubility values (thermodynamic activity) were calculated as described previously. A. 2. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. Published Dec 16, 2020 | Updated Dec 16, 2020. 6-, 12-, 24-, 48-, 96- and 384-well plates. g. 3. Linear, Orbital Slow, and Orbital Fast. edu, 814-863-3503. 1 software to identify the IC50. 306 plate air cal fail,low light: 316 grating motor driver faulty: 307 cuv air ref fail: 317 monitor ADC faultyThe BioTek Cytation 5 cell imaging multimode reader combines automated digital widefield microscopy with conventional multimode microplate reading. The EC50 values were calculated from the absorbance values vs. SpectraMax Injector Cartridge with SmartInject™ Technology. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. This unit must survive. 2. HTRF-compatible SpectraMax Multi-Mode Readers. The lower . Select the lab user profile. pH-Dependent Solubility. Turn on Plate Reader and wait for it to complete its start-up routine. Then, luminescence of samples was read in a Spectramax M5 plate reader with integration time of 5 s. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Also refer to this Multimode Reader Comparison Chart. 40266 Email: drugdiscoverytech@lifetech. Quick Guide to the plate reader. Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Detection modes are available in UV-Vis absorbance, fluorescence, and. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The password is. S. 1% Tween-20 and bound secondary was detected using Ultra TMB (Thermo Fisher); signals were read on a Molecular Devices Spectra Max M5. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. 4: Test tube cover. We use Spectramax M5 microplate reader in our lab. 025 fmol/well, 384-well plate) Quad monochromator: Fluorescein 2. Junichi Yamamoto a, Takumi Ito b, Yuki Yamaguchi a and Hiroshi Handa * c a School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan b Institute of Medical Science, Tokyo Medical. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. Microplate automation and detection instruments for walk away operation and high quality results. With SOFTmax PRO, the contents of the wells in a microplate can be mixedAdjusting fluorescence gain on microplate readers. Minimize manual manipulation of samples and data processing, and improve laboratory efficiency. 91 cm x 44. Microplate Capacity. Lucifer yellow was measured using an M5 plate reader (Molecular Probes) at an excitation wavelength of 485 nm and an emission wavelength of 530 nm. coli cells were purchased from New England Biolabs (Ipswitch, MA). To determine the DAR, STRO-002 in plasma samples were captured using Streptavidin Mag Sepharose Beads (GE Healthcare) coated with biotinylated. 3. Plate Analysis 9. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. The reaction was monitored with a SpectraMax M5 plate reader photometer (Molecular Devices GmbH, Biberach a. Briefly, Nunc microtiter plates were coated overnight at 2° to 8°C with KLH (3 μg/ml; IMMUCOTHEL, biosyn Arzneimittel GmbH) prepared in PBS. Other features. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. The inhibitory rate of ingredients was calculated as follows: Inhibition (%) = (FI of control – FI of treatment) / FI of control × 100. Finally, the absorbance of color development was read by SpectraMax M5 plate reader (Molecular Devices, San Jose, USA) at wavelength of 450 nm. Absorbance. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. The plates were centrifuged at the end of incubation, and fluorescent signals in supernatants were measured using a SpectraMax™ M5 microplate reader, with excitation at 485 nm and emission at 520 nm. 4: Test tube cover. Cultures were grown at 30°C and 900 rpm for 24 h, then OD600 was measured on a SpectraMax M5 plate reader to determine bacteriostatic potential. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. 1. Assay was run using JAK1 JH1/JH2 and Omnia Y peptide 12 kit. Then, absorbance was measured by a Spectra Max ™ M5 plate reader (Molecular Devices, Sunnyvale, CA). 2. Assay for Misfolded SOD1. reader. S6. After the plate is read, data will appear in the plate. ThT fluorescence was measured at 444 and. When the user applies a set temperature of 37°C, the top is heated to 37. 2. The threshold of detection was calculated as the mean plus three standard deviations of the luminescence from the supernatant of. The SpectraMax M2 and M2e are versatile and reliable. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. 1. 5. 2000) supplemented with each chemical com-pound with the final concentration of 10 mmol l-1. You can also interpret your results using integrated data analysis software. 1 g/ml SDS solution in 0. Fuchs1,2,3* 1 Department of Biology, Tufts University, Medford, MA 02155, USA 2 Institute for Protein Innovation, Boston, MA 02115, USA 3 Program in Cellular and Molecular Medicine,. The reduced readings obtained from the plate reader were analyzed using PRISM 6. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. The percent relative binding potency was calculated by dividing the reference material. were read using SpectraMax M5 plate reader (Molecular Devices, San Jose, CA, USA) with excitation of 380 nm and emission of 460 nm. Cell apoptosis assay. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:FlexStation® 3 Multi-Mode Microplate Reader. Maximum signal with lowest background. Turn the reader on and secure the USB cable. PURPOSE. 5: Schematic of the back panel of a reader. All enzymes used for DNA manipulations were purchased from New. Fast protein liquid chromatography (FPLC) was performed as the last protein purification step on a Bio-Rad BioLogic DuoFlow using a HighPrep TM 26/60 Sephacryl TM S-200 High Resolution column. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. was measured atdetermined450 nm using SpectraMax M5 plate reader. Uncontrolled Copy for Training and Reference Purposes Only . Find regional support. Five preferred detection modes. 500 mm/s is the maximum speed of the extruder of AnkerMake M5 when using Fast Mode. Kinetic data for Omnia® assays on the SpectraMax® M5/M5e reader. Shop now. 2% acetamide. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. The absorbance was read at 450 nm with 650 nm as background correction using Softmax software on M5 plate reader (Molecular Devices). 1. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. The fluorescent intensity of the cells reflecting the LIP was quantified by measuring the fluorescent signal at excitation wavelength (λex) of 543 nm and emission wavelength (λem) of 580 nm as per the manufacturer recommendation using a SpectraMax M5 plate reader (Molecular Devices) and normalized to the cell count. Open and turn on the laptop. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. The decrease in absorbance at 340 nm was analyzed for the 200 s of the reaction, which corresponds to the initial rate of the reaction. In general, reading fluorescence from the top is more sensitive than reading from the bottom. Monocytes were seeded in 24 or 96 well plates at 5 × 10 5 cells/mL and differentiated to macrophages for 7 days in X-VIVO 10 (Lonza) medium supplemented with 1% human serum as previously described . Even better, they require MINIMAL modification to. The spectrophotometric performance of the SpectraMax M2 and SpectraMax M2e is similar to the SpectraMax Plus, a dedicated absorbance plate reader. 5 cm) Weight 80 lbs (36. 5. 1, and the color is formed by the reaction in the microtiter plate. Most papers they cultured bacteria to OD600>0. The GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. 5 × 10 5 counts/fmol when measured with a Spectromax M5 plate reader using white opaque 96-well plates. Thank you!. The absorbance at a wavelength of 540 nm was quantified using a Spectramax M5 plate-reader (Molecular Devices) to determine the amount of gamma glutamyl hydroxamate, indicative of active enzyme. Flock Safety is an Atlanta-based company that sells automatic license plate detection readers, or ALPRs for short, to law enforcement agencies, schools, neighborhood homeowners associations and. For SpectraMax L or LMax II, LMax II 384 - no adapter used. Subsequently, 200 μL of filtrate was transferred into a 96-well UV Star microplate, and the protein concentration in each filtrate was determined using a SpectraMax M5 UV-Visible plate reader. FeCl 3, iron standards are always included (0-4 µg /ml). The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. xlsx file, with or without the averages and normalizations. 4 fmol/well, 384-well plate)−bottom Reading Speed (Kinetic) 96 wells: 11 s 384 wells: 22 s Luminescence Wavelength Range 300−700 nm Dynamic Range > 6 decades Sensitivity. Plate Reader (3) Name Price; EnSpire Flexstation 3 SpectraMax M5 Plate. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. 25 pM (0. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. 1. Cells were seeded at a density of 1,000 cells/well (A549: 500 cells/well). 7. EPOCH2 Plate Reader/Spectrophotometer. 320 nm and em = 456 nm) on a Spectramax M5 plate. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. com 10. SpectraMax M5 plate reader (Molecular Devices, San Jose, CA) was used. 2. Turn on the SpectraMax M5 Multi-Mode Microplate Reader. M-5 The M-5 multitronic unit, or the M-5 computer, was an advanced multitronic computer system and prototype created by Doctor Richard Daystrom during. There is currently no charge for using this plate reader. doi: 10. 9000-0756. Microplate Readers. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. One hundred microliters of the supernatant was then transferred into a 96-well plate and read in a SpectraMax M5 plate reader (Molecular Devices) at A570. If the computer is connected to the Internet: Select Search Automatically for updated driver software. 5 pM (0. Fluorescein 0. the contents were carefully mixed by pipetting up and down. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax M4 reader How does the reader perform a self check?. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Fluorescence Microplate Reader Comparison Chart. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models. STAKMAX. Chirag considers the SpectraMax M5 a multi-purpose reader that.